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Article Details ::
Article Name :
INTERGENERIC MICROBIAL CO-FLOCS: POSITIVE ROLE OF BIOTIC AND ABIOTIC FACTORS ON THE ENHANCEMENT OF CO-FLOCCULATION BETWEEN PGPR CELLS
Author Name :
Mahendran. R , Sekar. C
Publisher :
Ashok Yakkaldevi
Article Series No. :
ISRJ-341
Article URL :
Author Profile
Abstract :
The positive effect of certain biotic and abiotic factors viz., Inoculum level and growth phase, cultural condition, pH, presence of divalent cations and chelating agents on the enhancement of co-flocculation between PGPR cells viz., Pseudomonas fluorescens (PF-5) and Paenibacillus polymyxa (PB-5) was studied under in vitro condition. It was observed that 107:107 inoculum level and stationary growth phase of PGPR partners were found to be optimum for maximising the coflocculation. The cells of PGPR partners harvested from N-deficient medium recorded more co-flocculation when compared to PGPR cells harvested from N-rich medium. Further, The PGPR cells cultured at 350c and at pH level of 7.5 recorded higher co-flocculation percentage than other levels. Moreover, the addition of Ca2+, as divalent cations, augmented higher co-flocculation percentage of PGPR cells while the addition of EDTA, as chelating agent, reduced the stability of PGPR coflocs, significantly.
Keywords :
  • pgpr
  • biotic and abiotic factors
  • coflocculation
  • divalent cations
  • chelating agents